ABSTRACT
Background: Hypertension is a health problem and alternative medicine is offered for its controlling
Objective: Nutritional effects of Lavandula angustifolia L. [lavender] oil on high blood pressure [BP], and histological changes of the aorta in rat has been investigated in this study
Methods: Therty five male wistar rats were divided into 5 groups; normal, hypertensive and control. Hypertension was induced by daily L-NAME administration in drinking water for 6 weeks and lavender oil [500 mg/kg] or its vehicle was given orally simultaneously. After basal BP measurement, lavender [50 mg/kg, iv] was injected in control groups. Histological evaluation of the thoracic aorta included staining with hematoxylin-eosin and orcein methods
Results: Hypertension induction increased mean arterial BP from 106 to 177 mm Hg and lavender treatment reduced it to 140 mm Hg [P< 0.001]. Lavender had no effect on normal rats. Intravenous injection of lavender reduced BP about 75% in normotensive and hypertensive rats [P<0.001]. Hypertension induction increased cross-section area, aorta and media thickness, and reduced elastic lamina thickness [P<0.001]. Lavender treatment reduced cross-section area of the aorta and media thickness, and increased elastic lamina thickness [P < 0.05]. Hypertension and treatment had no effect on advanthia and elastic lamina numbers.
Conclusion: Nutritional lavender oil prevented BP increases and pathological changes of the aorta in hypertensive rats. It seems lavender oil is beneficial for prophylaxis of hypertension
ABSTRACT
Background and Objective: Several studies have shown that inhalation of Lavandula angustifolia L. [Lavender] reduces hypertension, while systemic effects and mechanism of action of lavender oil on blood pressure is not clear. This study was carried out to evaluate the effect of intravenous and ntraperitoneal injection of Lavandula angustifolia L. oil on normal blood pressure in male rats
Methods: In this experimental study, 70 male Wistar rats were randomly allocated into 10 groups [n=7]. Following anesthetizing the animals with sodium thiopental, femoral artery and vein were cannulated respectively for recording blood pressure and injection of Lavandula angustifolia L. oil. Lavender oil or its vehicle [Propylene glycol] was injected by intravenous [25, 50 and 100 mg/kg/bw] or intraperitoneal injection [500mg/kg/bw]. For the evaluation of the mechanism of Lavender oil, L-NAME [4mg/kg/bw], atropine [1mg/kg/bw], indomethacin [5 mg/kg/bw] or saline was injected intraperitoneally before intravenous administration of Lavender angustifolia L. oil
Results: Intravenous injection of Lavender oil of 25 and 50 mg/kg/bw reduced arterial blood pressure in compare to control group [P<0.05] and dose of 50 mg/kg/bw was more effective than dose of 25 mg/kg/bw [P<0.05]. 100 mg/kg/bw of Lavender angustifolia L. oil caused serious fall of blood pressure and resulted in animal death. Intraperitoneally injection of Lavender angustifolia L. oil at dose of 500 mg/kg reduced arterial blood pressure that this reduction was longer than intravenously administration of Lavender oil [P<0.05]. Intraperitoneal injection of L-NAME, atropine or indomethacin had no significant effect on baseline of blood pressure and hypotensive effect of Lavender angustifolia L. oil
Conclusion: Intravenous injection of Lavandula angustifolia L. oil in doses of 25 and 50 mg/kg/bw reduced arterial blood pressure in rat, but intraperitoneally injection of Lavender oil at dose of 500 mg/kg/bw prolonged the reduction of blood pressure in animals
Subject(s)
Animals, Laboratory , Plant Oils , Blood Pressure , Injections, Intravenous , Injections, Intraperitoneal , Rats, WistarABSTRACT
Ferula persica has been used in traditional medicine for treatment of high blood pressure. In this study acute and chronic effect of aqueous F. persica extract on BP of hypertensive rats and its possible mechanism of action have been investigated. Eighty two male Wistar rats were divided into 12 experimental groups. Hypertension was induced by Goldblatt method in the anesthetized rats. Aqueous extract of F. persica [15 or 30 or 60 mg/kg, iv] or it's vehicle were administered in treatments or control groups to evaluate their effects on BP and heart rate. To assess the mechanism of F. persica action on BP, L-NAME [5 mg/kg], Atropine [1 mg/kg] or Indomethacin [5 mg/kg] were injected intraperitoneally followed by intravenous administration of F. persica [30 mg/kg] in the different groups of hypertensive rats. Chronic effect of F. persica [30 mg/kg] on BP was evaluated by the aqueous extract administration in drinking water for a month. Intravenous administration of F. persica reduced BP of hypertensive rats [p<0.001]. There is no significant different between three doses of F. persica. Intraperitoneal injection of L-NAME, Atropine or Indomethacin has no significant effect on basal BP, but L-NAME eliminated and Atropine reduced hypotensive effect of F. persica extract on BP. Chronic administration of F. persica has no effect on BP. Our findings showed the hypotensive effect of F. persica in hypertensive rats may be mediated by muscarinic receptors and NO release
ABSTRACT
Background and Purpose: Cuminum Cyminm [CC] is one of the plants whose aqueous extract is used in traditional medicine as anti-convulsive, anti-epileptic and as a tretment for some visceral pains. However, no substantial reports can be found of its effects on the peripheral pain in lab animals. Therefore, this study was condcated to determine the eefects of the IP injection of the aqueous extract of CC on the acute peripheral pain in Hot Plate [HP] and Tail Flick [TF] tests in mice
Methods and Materials: This experimental study was conducted on 56 male Albino mice [8 groups each with 7 mice] weighing 25-30 gr. 200 and 500 mg/kg of the aqueous extract of CC or saline of the volume were injected intraperitoeally 30 minutes prior to the tests. Pain measrment criteria in the TF test was the response time to the pain by raising the tail, and in the HP test the time to begin to lick the front legs or raising the rear feet
Results: The IP injection of the CC aqueous extract with the doses of 200 and 500 mg/kg significantly increasd the response time to the pain in HP and TF tests [P<0.01] in cmparison with the control groups and the group receiving saline. The efect was however more remarkable with the 500 mg/kg dose
Conclusion: The results of this study indicated that the aqueous extract of CC reduced the acute peripheral pain in the mice
ABSTRACT
Background and Purpose: Previous studies suggest that the leaf of Trigonella foenum [TF] has analgesic, anti-inflammatory and probably modulatory effects on sleep and anxiety reactions. The present study is intended to determine the effects of hydro - alcoholic extract of TF on anxiety and sleeping time in mice
Methods and Materials: In this experimental study, 140 male albino mice [25-30 gr] were used in 14 groups [n=10 in each group]. Elevated plus maze [EPM] was used for the assessment of anxiety, and experimental groups received hydro-alcoholic extracts of TF [50, 100, 200, 400 and 80 mg/kg] as IP injection; sham group received saline [10 mg/kg IP injection] 30 minutes prior to evaluation; the control group did not receive any injection. 25 minutes after injection, animals were put in a black box for 5 minutes in order to increase their activity; then, they were transferred to the EPM for anxiety evaluation by two factors: the time spent and frequency of entrance to the open arms of the EPM. In order to assess sleeping time, the Angel method [records of sleeping duration by physiograph] was used. 30 minutes before sleep assessment, TF extract and saline were used for experimental and sham groups; the control group received nothing as what we did in anxiety evaluation. Finally, ANOVA and Tukey tests were used for data analysis
Results: The findings indicated that the hydro-alcoholic extract of TF in doses of 100 and 200 mg/kg significantly increased the time spent and frequency of entrance to the open arms of EPM [p<0.05] in the experimental groups rather than in sham and control groups; this indicates a reduction of anxiety. Also, the TF extract in doses of 200 and 400 mg/kg significantly increased sleep duration [p<0.05] in comparison with the sham and control groups
Conclusion: The results indicated that the TF extract had an effective role in reducing anxiety reactions and increasing sleep duration in mice
ABSTRACT
Anxiety is one of the most common psychotic disorders. Since available antianxiety therapies have many side effects, searching for new drugs with lower side effects is necessary. Achillea. Millefolium is one of the medical plants that have many beneficial effects and it seems that it has some anti-anxiety effects as well. In the current study, the probable influence of hydro-alcholic extract of A. Millefolium on anxiety-like behaviors was investigated in a mice model. Sixty male albino mice [25-30gr] were used in the study. Animals were randomly divided into four treatment groups and two control groups. The treatment groups were received different doses of A. Millefolium extract [50, 100, 200 and 400 mg/kg] by the route of intraperitoneal. One of the control groups was given the vehicle of A. Millefolium extract, while another one; sham control, received only a blank injection. Twenty-five min following injection of the A. Millefolium extract, the animals have left inside a black wall box for increasing motion activity for 5 min. Then the animals were transferred to a plus maze for the evaluation of anxiety reaction including the number of entries and time spent in the open arms of maze. These anxiety criteria were monitored and recorded for 5 min. The hydroalcohlic extract of A. Millefolium in a dose of 100, 200 and 400 mg/kg significantly increased both the time spent in the open arms and the number of entries to the open arms in the test animals compared to the control animals [p<0.05]. The results of present study showed that A. Millefolium extract modulates anxietylike behaviors in mice